Nano-formulation for topical treatment of precancerous lesions: skin penetration, in vitro, and in vivo toxicological evaluation
- Calienni, Maria Natalia
- Temprana, Carlos Facundo
- Prieto, Maria Jimena
- Paolino, Donatella
- Fresta, Massimo
- Tekinay, Ayse Begum
- Alonso, Silvia del Valle
- Martinetti Montanari, Jorge Anibal
DOIAbstract
With the aim of improving the topical delivery of the antineoplastic drug 5-fluorouracil (5FU), it was loaded into ultradeformableliposomes composed of soy phosphatidylcholine and sodium cholate (UDL-5FU). The liposome populations had a mean size of70 nm without significant changes in 56 days, and the ultradeformable formulations were up to 324-fold more elastic thanconventional liposomes. The interaction between 5FU and the liposomal membrane was studied by three methods, and alsorelease profile was obtained. UDL-5FU did penetrate the stratum corneum of human skin. At in vitro experiments, the formulation was more toxic on a human melanoma-derived than on a human keratinocyte-derived cell line. Cells captured liposomes bymetabolically acti...
Add to ORKG