Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens
- Adler, Conrado
- Michavila, Gabriela
- Lopez, Fabian Enrique
- Corbalan, Natalia Soledad
- Lami, María Jesús
- Filippone, María Paula
- Castagnaro, Atilio Pedro
- Vincent, Paula Andrea
- de Cristobal, Ricardo Ezequiel
DOIAbstract
This work identifies a compound able to inhibit Xanthomonas citri subsp. citri(citrus canker agent) growth in vitro and in vivo. First, we isolated bacteria fromcitrus leaf surfaces that were able to inhibit X. citri subsp. citri in vitro. Among theselected isolates, we focused on one with remarkable activity. The strain wascharacterized as Pseudomonas fluorescens after sequencing its 16S rDNA andanalyzing the sequence with BLASTn. The purification and chemical analysis of theactive compound allowed us to assign the inhibitory activity to enantio-pyochelin (EPch).Since this molecule is a siderophore, we wondered if the inhibition observedwas a result of iron scavenging. Surprisingly, when we supplemented media with anexcess of iron, we obse...
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