Associated fungi, symbiotic germination and in vitro seedling development of the rare Andean terrestrial orchid Chloraea riojana
- Fracchia, Sebastian
- Aranda Rickert, Adriana Marina
- Rothen, Carolina Paola
- Sede, Silvana Mabel
DOIAbstract
Chloraea riojana is a rare terrestrial orchid from the Andean region of Northwest Argentina. Here we evaluate the mycorrhizal status, symbiotic germination and protocorm development of the species. Plant material including capsules, roots and whole plant were collected from two natural populations. Symbiotic germination was tested using one fungal endophyte isolated from C. riojana and four fungal endophytes previously isolated from the sympatric orchid species Aa achalensis. Three isolates were able to promote seed germination and further development into plantlets with 1?2 leaves after 16 weeks of in vitro dual culture. Moreover, the strain isolated from C. riojana developed intense melanized coilings and pelotons inside protocorms, a rar...
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