Epidermal growth factor receptor silencing blunts the slow force response to myocardial stretch
- Brea, María Soledad
- Diaz, Romina Gisel
- Escudero, Daiana Sabrina
- Caldiz, Claudia Irma
- Portiansky, Enrique Leo
- Morgan, Patricio Eduardo
- Perez, Nestor Gustavo
DOIAbstract
Background-Myocardial stretch increases force biphasically: the Frank-Starling mechanism followed by the slow force response (SFR). Based on pharmacological strategies, we proposed that epidermal growth factor (EGF) receptor (EGFR or ErbB1) activation is crucial for SFR development. Pharmacological inhibitors could block ErbB4, a member of the ErbB family present in the adult heart. We aimed to specifically test the role of EGFR activation after stretch, with an interference RNA incorporated into a lentiviral vector (small hairpin RNA [shRNA]-EGFR). Methods and Results-Silencing capability of p-shEGFR was assessed in EGFR-GFP transiently transfected HEK293T cells. Four weeks after lentivirus injection into the left ventricular wall of Wista...
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