Add to ORKGEncapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing ½ MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for 1 h, thawed at 30 °C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on ½ MS med...
Miltonia flavescens is a species vulnerable to extinction, which justifies research on preservation ...
Thailand is home to a variety of wild orchids, most of which grow on trees in the forests. However, ...
Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsul...
Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of ...
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration tech...
The development of a long-term conservation method is crucial for orchid germplasm preservation and ...
In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid were cryopr...
Protocorm-like bodies (PLBs) of Brassidium Shooting Star, a new commercial ornamental orchid hybrid,...
In vitro grown protocorm-like bodies (PLBs) of Dendrobium Bobby Messina hybrid were cryopreserved in...
A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot ...
In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid were cryopreserved...
© 2015, The Society for In Vitro Biology. In a bid to better conserve endangered terrestrial orchids...
In the present studies, an attempt has been made to develop a method of liquid nitrogen preservation...
This study was carried out to evaluate the potential of using the encapsulation-dehydration techniqu...
Cryopreservation of apices sampled on in vitro plantlets of #Poncirus trifoliata$ (L.) Raf. was achi...
Miltonia flavescens is a species vulnerable to extinction, which justifies research on preservation ...
Thailand is home to a variety of wild orchids, most of which grow on trees in the forests. However, ...
Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsul...
Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of ...
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration tech...
The development of a long-term conservation method is crucial for orchid germplasm preservation and ...
In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid were cryopr...
Protocorm-like bodies (PLBs) of Brassidium Shooting Star, a new commercial ornamental orchid hybrid,...
In vitro grown protocorm-like bodies (PLBs) of Dendrobium Bobby Messina hybrid were cryopreserved in...
A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot ...
In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid were cryopreserved...
© 2015, The Society for In Vitro Biology. In a bid to better conserve endangered terrestrial orchids...
In the present studies, an attempt has been made to develop a method of liquid nitrogen preservation...
This study was carried out to evaluate the potential of using the encapsulation-dehydration techniqu...
Cryopreservation of apices sampled on in vitro plantlets of #Poncirus trifoliata$ (L.) Raf. was achi...
Miltonia flavescens is a species vulnerable to extinction, which justifies research on preservation ...
Thailand is home to a variety of wild orchids, most of which grow on trees in the forests. However, ...
Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsul...