Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine

The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8+ CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8+ T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8+ T cells expands following multiple immunizations with this vaccine.Fil: Pedersen, Lasse E.. United States Department of Agriculture. Agricultural Research Service; Argentina. Technical University of Denmark; DinamarcaFil: Patch, Jared R.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados UnidosFil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service; ArgentinaFil: Glabman, Raisa A.. United States Department of Agriculture. Agricultural Research Service; Argentina. University of Vermont; Estados UnidosFil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Jungersen, Gregers. Technical University of Denmark; DinamarcaFil: Buus, Soren. Universidad de Copenhagen; DinamarcaFil: Golde, William T.. United States Department of Agriculture. Agricultural Research Service; Argentin