Donor Mesenchymal Stromal Cells (MSCs) Undergo Variable Cardiac Reprogramming in Vivo and Predominantly Co-Express Cardiac and Stromal Determinants after Experimental Acute Myocardial Infarction

We previously showed the emergence of predominantly non-fused murine cells co-expressing cardiac and stromal determinants in co-cultures of murine mesenchymal stromal cells (MSCs) and rat embryonic cardiomyocytes. To determine whether a similar phenotype is detectable in vivo in ischemic myocardium, we infused green fluorescence protein (GFP)-marked MSCs intravenously into wild-type mice in an acute myocardial infarction (AMI) model generated by ischemia/reperfusion (I/R) or fixed coronary artery ligation. We found that infused GFP+ cells were confined strictly to ischemic areas and represented approximately 10% of total cellularity. We showed that over 60% of the cells co-expressed collagen type IV and troponin T or myosin heavy chain, characteristic of MSCs and cardiomyocytes, respectively, and were CD45(-). Nonetheless, up to 25% of the GFP+ donor cells expressed one of two cardiomyocyte markers, either myosin heavy chain or troponin T, in the absence of MSC determinants. We also observed a marked reduction in OCT4 expression in MSCs pre-infusion compared with those lodged in the myocardium, suggesting reduced stem cell properties. Despite the low frequency of lodged donor MSCs, left-ventricular end-diastolic pressure was significantly better in experimental versus saline animals for both AMI (12.10±1.81 vs. 20.50±1.53 mmHg, p=0.001) and I/R models (8.75±2.95 vs. 17.53±3.85 mmHg, p=0.004) when measured 21 days after MSC infusion and is consistent with a paracrine effect. Our data indicate that donor MSCs undergo variable degrees of cardiomyocyte reprogramming with the majority co-expressing cardiomyocyte and stromal markers. Further studies are needed to elucidate the factors mediating the extent of cardiomyocyte reprogramming and importance of the cellular changes on tissue repair.Fil: Yannarelli, Gustavo Gabriel. University of Toronto; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tsoporis, James N.. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; CanadáFil: Desjardins, Jean Francois. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; CanadáFil: Wang, Xing Hua. University of Toronto; CanadáFil: Pourdjabbar, Ali. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; CanadáFil: Viswanathan, Sowmya. University of Toronto; CanadáFil: Parker, Thomas G.. St. Michael’s Hospital. Li Ka Shing Knowledge Institute. Keenan Research Centre. Department of Medicine. Division of Cardiology; CanadáFil: Keating, Armand. University of Toronto; Canad