Glycosylation-dependent binding of galectin-8 to activated leukocyte cell adhesion molecule (ALCAM/CD166) promotes its surface segregation on breast cancer cells

Fernández, Marisa Mariel
Ferragut, Fátima
Cardenas Delgado, Victor Manuel
Bracalente, María Candelaria
Bravo, Alicia Ines
Cagnoni, Alejandro
Nuñez, Myriam Carmen
Morosi, Luciano Gastón
Quintá, Héctor Ramiro
Espelt, Maria Victoria
Troncoso, María Fernanda
Wolfenstein, Carlota Elisa
Mariño, Karina Valeria
Malchiodi, Emilio Luis
Rabinovich, Gabriel Adrián
Elola, Maria Teresa

Open link

Publication date

April 2016

DOI

10.1016/j.bbagen.2016.04.019.

Publisher

Elsevier BV

Journal

Biochimica et Biophysica Acta (BBA) - General Subjects

Abstract

We previously demonstrated that the activated leukocyte cell adhesion molecule (ALCAM/CD166) can interact with galectin-8 (Gal-8) in endothelial cells. ALCAM is a member of the immunoglobulin superfamily that promotes homophilic and heterophilic cell-cell interactions. Gal-8 is a "tandem-repeat"-type galectin, known as a matricellular protein involved in cell adhesion. Here, we analyzed the physical interaction between both molecules in breast cancer cells and the functional relevance of this phenomenon.Fil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Fa...