First detection of hepatitis E virus in Central Argentina: Environmental and serological survey

Background: The hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis worldwide, transmitted by fecal-oral route. In Argentina it is considered rare, so differential laboratory testing is not routinely performed. Besides, in Argentina's central area epidemiological and molecular characteristics of HEV are still unknown. Objectives: Provide evidence of local circulation of HEV by molecular detection on environmental samples and by serological survey in healthy adult population of Córdoba city, Argentina. Study design: Environmental surveillance was conducted in river and sewage samples collected between 2007 and 2009–2011. Viral detection was performed by RT-Nested PCR of ORF-1 and ORF-2 partial regions. Anti-HEV IgG was determined by EIA in 433 serum samples collected between 2009 and 2010. Results: HEV was detected in 6.3% of raw sewage samples and in 3.2% of riverine samples. Nucleotide sequencing analyses revealed that all isolates belonged to genotype 3, subtypes a, b and c. The prevalence of IgG anti-HEV was 4.4%. Seroprevalence increased with the age of the individuals (OR: 3.50; 95% CI 1.39–8.87; p = 0.0065) and, although the prevalence was higher in low income population, no statistical relation was found between anti-HEV and socioeconomic level. Conclusions: The environmental findings added to serological results, demonstrate that HEV circulates in central Argentina. Contamination of water with HEV could represent a route of transmission for local populations, which have a high number of susceptible individuals. This fact alerts local health care systems in order to include detection of HEV in the diagnostic algorithm of viral hepatitis.Fil: Martinez Wassaf, Maribel Graciela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Laboratorio LACE; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barril, Patricia Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Elbarcha, Osvaldo C.. Laboratorio LACE; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Pinto, Marcelo A.. Instituto Oswaldo Cruz; BrasilFil: Oliveira, Jaqueline Mendes de. Instituto Oswaldo Cruz; BrasilFil: DiGiusto, Pablo. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; ArgentinaFil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; ArgentinaFil: Ré, Viviana Elizabeth. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin