Agrobacterium tumefaciens-Mediated Transient Transformation of Arabidopsis thaliana Leaves

Mangano, Silvina
Gonzalez, Cintia Daniela
Petruccelli, Silvana

Open link

Publication date

January 2014

DOI

10.1007/978-1-62703-580-4_8

Publisher

Springer Science and Business Media LLC

ISSN

1064-3745

Journal

1064-3745

Citation count (estimate)

Abstract

Transient assays provide a convenient alternative to stable transformation. Compared to the generation of stably transformed plants, agroinfi ltration is more rapid, and samples can be analyzed a few days after inocu-lation. Nevertheless, at difference of tobacco and other plant species, Arabidopsis thaliana remains recalci-trant to routine transient assays. In this chapter, we describe a transient expression assay using simple infi ltration of intact Arabidopsis leaves with Agrobacterium tumefaciens carrying a plasmid expressing a reporter fl uorescent protein. In this protocol, Agrobacterium aggressiveness was increased by a prolonged treatment in an induction medium defi cient in nutrients and containing acetosyringone. Besides, Arabidop...