Stabilization of polymer lipid complexes prepared with lipids of lactic acid bacteria upon preservation and internalization into eukaryotic cells
- Alves, P.A
- Hugo, Ayelen Amelia
- Szymanowski, Felipe
- Tymczyszyn, Emma Elizabeth
- Perez, Pablo Fernando
- Cohelo, J.F.J.
- Simoes, P.N
- Gomez Zavaglia, Andrea
DOIAbstract
The physicochemical characterization of polymer liposome complexes (PLCs) prepared with lipids of lactic acid bacteria and poly(N,N-dimethylaminoethyl methacrylate) covalently bound to cholesterol (CHO-PDMAEMA) was carried out in an integrated approach, including their stability upon preservation and incorporation into eukaryotic cells. PLCs were prepared with different polymer:lipid molar ratios (0, 0.05 and 0.10). Zeta potential, particle size distribution and polydispersity index were determined. The optimal polymer:lipid ratio and the stability of both bare liposomes and PLCs were evaluated at 37 °C and at different pHs, as well as after storage at 4 °C, −80 °C and freeze-drying in the presence or absence of trehalose 250 mM. Internaliz...
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