Mouse sperm begin to undergo acrosomal exocytosis in the upper isthmus of the oviduct
- la Spina, Florenza Antonella
- Puga Molina, Lis del Carmen
- Romarowski, Ana
- Vitale, Alejandra Mariel
- Falzone, Tomas Luis
- Krapf, Dario
- Hirohashi, Noritaki
- Buffone, Mariano Gabriel
DOIAbstract
Recent evidence demonstrated that most fertilizing mouse sperm undergo acrosomal exocytosis (AE) before binding to the zona pellucida of the eggs. However, the sites where fertilizing sperm could initiate AE and what stimuli trigger it remain unknown. Therefore, the aim of this study was to determine physiological sites of AE by using double transgenic mouse sperm, which carried EGFP in the acrosome and DsRed2 fluorescence in mitochondria. Using live imaging of sperm during in vitro fertilization of cumulus-oocyte complexes, it was observed that most sperm did not undergo AE. Thus, the occurrence of AE within the female reproductive tract was evaluated in the physiological context where this process occurs. Most sperm in the lower segments ...
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