Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
- Morvillo, V.
- Luthy, Isabel Alicia
- Bravo, A. I.
- Capurro, M. I.
- Portela, P.
- Calandra, Ricardo Saul
- Mordoh, Jose
DOIAbstract
The presence and characteristics of androgen receptors (AR) have been described by our group in one human melanoma cell line. We now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies of human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein for IIB-MEL-LES cells and of 14 nM with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN. When specificity was assessed, as seen before, not only androgen and antiandrogen, but also non-androgenic compounds were able to compete for [3H]-R1881 bind...
Add to ORKG