Add to ORKGDNA polymerases have recently emerged as important cellular targets for chemical intervention in the development of anti-cancer agents. This report describes a PCR assay as a method to investigate the action mechanism of the inhibition of Taq DNA polymerase by catalpol. This inhibition was not primer or template specific, nor was it due to chelation of Mg2+ ions. In assays of hyperchromicity of double-stranded DNA, catalpol did not affect melting profile. The inhibitory effect of catalpol does not appear to depend on DNA concentration. In contrast, increasing dNTP concentration rescue the Taq DNA polymerase activity, suggestingthat catalpol acts in a competitive way with dNTPs at the binding site of the enzyme. Theoretical calculations rein...
Human DNA polymerase kappa (pol k) is a translesion synthesis (TLS) polymerase that catalyzes TLS pa...
In order to understand further the molecular mode of action of 5-Aza-2’-deoxycytidine (5-AZA-dCyd), ...
Taq DNA polymerase from Thermus aquaticus is used in the polymerase chain reaction. Not only is Taq ...
All living cells perform DNA replication through a group of enzymes called DNA polymerases. Unfortun...
<div><p>Human DNA polymerase kappa (pol κ) is a translesion synthesis (TLS) polymerase that catalyze...
Human DNA polymerase kappa (pol κ) is a translesion synthesis (TLS) polymerase that catalyzes TLS pa...
Faithful replication of DNA molecules by DNA polymerases is essential for genome integrity and corre...
Prior to the discovery of a thermostable Taq polymerase, anyone conducting a PCR reaction procedure ...
Using a feasible method, we generated a small focused library of structurally related alkenylcoumari...
<p>(A) A console outcome of qPCR using various concentrations of dNTP: 1, 1000 μM; 2, 500 μM; 3, 200...
Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquat...
The cellular genome is constantly subject to DNA damage caused by endogenous factors or exogenously ...
DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules...
Chromosomal duplication is targeted by various chemotherapeutic agents for the treatment of cancer. ...
<p>(A) Overview of a strategy for using DNA polymerases as signal recording devices. Signals (top) a...
Human DNA polymerase kappa (pol k) is a translesion synthesis (TLS) polymerase that catalyzes TLS pa...
In order to understand further the molecular mode of action of 5-Aza-2’-deoxycytidine (5-AZA-dCyd), ...
Taq DNA polymerase from Thermus aquaticus is used in the polymerase chain reaction. Not only is Taq ...
All living cells perform DNA replication through a group of enzymes called DNA polymerases. Unfortun...
<div><p>Human DNA polymerase kappa (pol κ) is a translesion synthesis (TLS) polymerase that catalyze...
Human DNA polymerase kappa (pol κ) is a translesion synthesis (TLS) polymerase that catalyzes TLS pa...
Faithful replication of DNA molecules by DNA polymerases is essential for genome integrity and corre...
Prior to the discovery of a thermostable Taq polymerase, anyone conducting a PCR reaction procedure ...
Using a feasible method, we generated a small focused library of structurally related alkenylcoumari...
<p>(A) A console outcome of qPCR using various concentrations of dNTP: 1, 1000 μM; 2, 500 μM; 3, 200...
Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquat...
The cellular genome is constantly subject to DNA damage caused by endogenous factors or exogenously ...
DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules...
Chromosomal duplication is targeted by various chemotherapeutic agents for the treatment of cancer. ...
<p>(A) Overview of a strategy for using DNA polymerases as signal recording devices. Signals (top) a...
Human DNA polymerase kappa (pol k) is a translesion synthesis (TLS) polymerase that catalyzes TLS pa...
In order to understand further the molecular mode of action of 5-Aza-2’-deoxycytidine (5-AZA-dCyd), ...
Taq DNA polymerase from Thermus aquaticus is used in the polymerase chain reaction. Not only is Taq ...