Microvesicle release and micellar attack as the alternative mechanisms involved in the red-blood-cell-membrane solubilization induced by arginine-based surfactants
- Fait, María Elisa
- Hermet, Melisa
- Comelles, Francesc
- Clapés, Pere
- Alvarez, Hugo Ariel
- Prieto, Eduardo Daniel
- Herlax, Vanesa Silvana
- Morcelle del Valle, Susana Raquel
- Bakas, Laura Susana
DOIAbstract
Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air-liquid interface, and the area per molecule indicated better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCnvia atomic-force microscopy supported the predictions based on the value of the surfactant-packing parameter (SPP). Erythrocyte-membrane solubilization was effected by surfactant aggregates since cell lysis became evident at only surfactant concentrations above the CMC. Changes in HRBC shape obser...
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