SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System
- Daza Millone, Maria Antonieta
- Ramirez, Eduardo Alejandro
- Chain, Cecilia Yamil
- Crivaro, Andrea Natalia
- Romanin, David Emmanuel
- Rumbo, Martín
- Docena, Guillermo H.
- Cocco, Mauro Daniel
- Pedano, Maria Laura
- Fainstein, Alejandro
- Montoya, Jorgelina Ceferina
- Vela, Maria Elena
- Salvarezza, Roberto Carlos
DOIAbstract
Surface Plasmon Resonance assays are being developed as alternative biodetection methods for a great number of pesticides and toxins. These substances typically have low molecular weight, making it necessary to perform competitive inhibition immunoassays. In most of the cases, the strategy is to immobilize a protein derivative of the analyte, which usually involves the appearance of nonspecific protein binding which limits the detection range of the assay. In this work we present results of a poly-L-lysine (Au-MUA-PLL) based sensor platform for quantitative determination of 2,4-dinitrophenol as model system for small molecular weight substances detection. The prepared sensor chip was characterized by means of Atomic Force Microscopy, Surfac...
Add to ORKG