Mapping the dynamical organization of the cell nucleus through fluorescence correlation spectroscopy
- Stortz, Martin Dario
- Angiolini, Juan Francisco
- Mocskos, Esteban Eduardo
- Wolosiuk, Alejandro
- Pecci, Adali
- Levi, Valeria
DOIAbstract
The hierarchical organization of the cell nucleus into specialized open reservoirs and the nucleoplasm overcrowding impose restrictions to the mobility of biomolecules and their interactions with nuclear targets. These properties determine that many nuclear functions such as transcription, replication, splicing or DNA repair are regulated by complex, dynamical processes that do not follow simple rules. Advanced fluorescence microscopy tools and, in particular, fluorescence correlation spectroscopy (FCS) provide complementary and exquisite information on the dynamics of fluorescent labeled molecules moving through the nuclear space and are helping us to comprehend the complexity of the nuclear structure. Here, we describe how FCS methods can...
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