Studies of water-soluble extract of Labisia Pumila var Alata of selected geographic origins

Labisia pumila(Myrsinaceae)or“kacip Fatimah”,is traditionally used in Malay traditional medicine in the form of decoction as postpartum tonic.Today,it is sold as functional food and beverage in response to consumer interest in its potential health benefits.However, nutrient data on this plant,for example,remains scarce.The aim of this research project was to determine the properties and attributes of water-soluble extracts of Labisia pumila var. alata to reveal its potential health benefits as functional food and beverage.The nutrient composition,total phenolic content,total flavanoid content and antioxidant activities of LP of various sources,viz.,Malaysian and Indonesian origins,were studied to determine the source with specific properties and attributes.PLS and PCA were then used to classify and to understand the correlation between total solid,total acidity,and solubility in cold and hot water, proximate properties,minerals,heavy metals,total phenolic content,and antioxidant activities of Labisia pumila var. alata leaves(LP)due to geographic origin.Based on results obtained,there were no significant difference in yield, total solid,total acidity,and solubility in cold and hot water of lyophilized LP extract due to geographic origin (p>0.05).The extract from Halimun-Salak Mountain,Bogor(LPB) was found to have the highest pH content.LPT was found to have the highest gallic acid value(1.86%),total phenolic content(140.49 mg GAE/g lyophilized plant material), antioxidant activity(FRAP) (17.57 mg GAE/g lyophilized plant material),DPPH(EC50)(78.79 µg/mL),protein (9.87±0.18 %), moisture (10.11±0.06 %), mineral Na (20.20 mg/100 g), K (1128 mg/100 g),Mg(476.1 mg/100g),and Zn (2.32 mg/100 g). The highest values of Ca and Fe were for LP from Pahang (LPR) at 265.8 mg/100g Ca and 41.61 mg/100g Fe, respectively.There was a significant difference(p