Production of glucose from banana stem waste by using Strain B

Banana is a major cash crop of many regions generating good amount of waste after harvest. This agricultural waste used as substrate for the fermentation process to produce organic products such ethanol and acetic acid. Production of glucose from agro waste is one way to reduce the abundant of waste on the plantation floor. The objectives of this research are to study the effect of organic loading rate (OLR) during glucose production, to optimize the glucose production by using banana stem waste and to study the suitability of banana stem waste as substrate for glucose production. In this study, eight experimental runs were carried out simultaneously at OLRs of 30, 5.0, 5.0, 17.50, 23.75, 11.25, 17.50 and 30 g/L.d respectively which have the same hydraulic retention times, three days. The effect of OLR was measured by total average yield of glucose produced for each run while the optimization of glucose production was done by using One Factor Analysis. Then, the suitability of banana stem in producing glucose was determined by doing comparison with other lignocelullose waste. The results reveal that, the OLR was affected to the glucose production. At range 5-23.75 g/L.d the yield was decreased as the OLR increased while at 30 g/L.d which the highest OLR the yield of glucose was higher compare to 23.75 g/L.d. This was possible due to the accumulation of inhibitor in the high influent substrate concentration. As the OLR increase, the accumulation of inhibitor increased. At 5 g/L.d, those OLR was selected as the optimum OLR for this study by using One Factor Analysis due to the maximum glucose production within thirty days experiment. As conclusion, banana stem waste was successfully degraded by strain B and produced glucose