Encapsulation of plant extracts containing phenolic compounds into nanoparticles and nanofibers

The presented bachelor thesis was focused on the encapsulation of extracts containing phenolic substances in order to apply these materials in cosmetics. At work were prepared aqueous, ethanol and lipid extracts of coffee and cocoa. All extracts were characterized on the content of all polyphenols and flavonoids. Their antioxidant aktivity and SPF were also determined. Then the selected extracts were encapsulated into liposomes and polyhydroxybutyrate nanofibres. The basic characteristics of the prepared liposomes were also observed and also their SPF and antioxidant aktivity were monitored. The highest antioxidant aktivity and SPF had liposome particles containing ethanol cocoa extracts. The liposome particles were applied to cosmetic emulsion. These creams evince high antioxidant acitivity and stability determined by analytical centrifugation. Also the prepared nanofibres had high antioxidant aktivity and confirmed the gradual release of the active substances. In conclusion the test cytotoxicity on human keratinocytes confirmed the safety of prepared nanomaterials using the MTT test, which are therefore suitable for cosmetic applications