This work deals with design of communication protocol for data transmission between control computer and computing cores implemented on FPGA chips. The purpose of the communication is speeding the performance demanding software algorithms of non-stream data processing by their hardware computation on accelerating system. The work defines a terminology used for protocol design and analyses current solutions of given issue. After that the work designs structure of the accelerating system and communication protocol. In the main part the work describes the implementation of the protocol in VHDL language and the simulation of implemented modules. At the end of the work the aplication of designed solution is presented along with possible extensio...