Add to ORKGThe monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot hybridization and in RNase protection assays is described. The relative advantages and pitfalls of these two methods to quantitatively detect mRNA targets are discusse
We have developed a simple method to measure RNA synthesis in real time. In this technique, transcri...
Since its development in 1983 (1), Ribonuclease Protection Assay (RPA) has become a widely employed ...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2005.Title supplied by cat...
The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unl...
Sequence-specific hybridization probes of high specific activity are prepared by cloning the probe s...
In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegan...
A simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure ...
High yields of RNA (e.g., mRNA, gRNA, lncRNA) are routinely prepared following a two-step approach: ...
AbstractA model for virion RNA of the poliomyelitis virus, which does not pass the stage of DNA copi...
Microarrays have established as instrumental for bacterial detection, identification, and genotyping...
An important problem often faced in the molecular characterization of genes is the precise mapping o...
<p>(A) Electron micrographs of intact chromosomes extracted from cells provide images of transcribin...
In the middle of the 1990's, Dr Jaw Ming Cherng, a previous student in the laboratory discovered th...
We present the detection of native mRNA using the BIACORE system. The influence of different probes ...
The evidence recently presented of specific hybridization between bacterial ribosomal RNA and homolo...
We have developed a simple method to measure RNA synthesis in real time. In this technique, transcri...
Since its development in 1983 (1), Ribonuclease Protection Assay (RPA) has become a widely employed ...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2005.Title supplied by cat...
The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unl...
Sequence-specific hybridization probes of high specific activity are prepared by cloning the probe s...
In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegan...
A simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure ...
High yields of RNA (e.g., mRNA, gRNA, lncRNA) are routinely prepared following a two-step approach: ...
AbstractA model for virion RNA of the poliomyelitis virus, which does not pass the stage of DNA copi...
Microarrays have established as instrumental for bacterial detection, identification, and genotyping...
An important problem often faced in the molecular characterization of genes is the precise mapping o...
<p>(A) Electron micrographs of intact chromosomes extracted from cells provide images of transcribin...
In the middle of the 1990's, Dr Jaw Ming Cherng, a previous student in the laboratory discovered th...
We present the detection of native mRNA using the BIACORE system. The influence of different probes ...
The evidence recently presented of specific hybridization between bacterial ribosomal RNA and homolo...
We have developed a simple method to measure RNA synthesis in real time. In this technique, transcri...
Since its development in 1983 (1), Ribonuclease Protection Assay (RPA) has become a widely employed ...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2005.Title supplied by cat...