Stimulating Effect of Mercuric Chloride and Silver Nitrate on the Dark Germination of Pine Seeds (4)

The growth inhibitors in Pinus densiflora seed were tested by the activity of oat coleoptile straight growth in relation to the breaking of dormancy with silver nitrate. Methanol extract of the seed, developed by paper chromatography with isopropanol・ammonia, showed in the zone of Rf 0.1～0.2 the positive difference only between the 'intact seed'treated with silver nitrate and the not-treated one, but it did not show the difference in the 'seed coats'. Ethylacetate partition of the methanol extract showed the inhibitor zone in Rf 0.8～0.9. The inhibitory action of Rf 0.8～0.9 on oat coleoptile growth was released with silver nitrate by floating filter paper sections of the zone in the solution though the seed inhibitor was contaminated with the filter paper inhibitor. The inhibitor zone of paper chromatograms was always coloured yellow or orange red with bis-diazotized benzidine.アカマツ種子の成長抑制物質を硝酸銀による休眠打破と関連づけて，アベナ伸長成長活性によってテストした。硝酸銀処理および無処理種子のメタノール抽出物をイソプロパノール・アンモニアによって展開したペーパークロマトグラムを用いては“完全種子”でRf0.1～0.2に差が見出されたが，種皮についてはその差が明らかでなかった。メタノール抽出液を醋酸エチルでふり直すと，Rf0.8～0.9に抑制帯を示し，その部分の浸出物質は〓紙に存在する抑制物質も含むが，硝酸銀によってアベナ成長に対する抑制作用を緩和する。また，ペーパークロマトグラムの抑制帯は，常にヂアゾ化したベンチジンによって黄色または橙赤色に発色する