Isolation and identification of a-proteobacteria from Culex pipiens (Diptera Culicidae) larvae
- Tranchida, Maria Cecilia
- Riccilo, P. M.
- Rodriguero, Marcela Silvina
- García, J. J.
- Micieli, Maria Victoria
DOIAbstract
A survey of drainage ditches in suburban areas of La Plata, Buenos Aires province, Argentina for pathogens of Culex pipiens larvae was conducted from 2003 to 2006. C. pipiens larvae of opaque, white color were found in several of those field collections. When the white larvae were dissected and observed by phase-contrast microscopy in wet-mount preparations, the presence of bacteria, located in the hemocoel, was recorded. Laboratory experiments were performed to elucidate the pathway for transmission of this pathogen. Although approaches involving traditional culturing had failed to reveal the identity of the new microorganism present, molecular techniques to identify the pathogen in the studies reported here were successful. The partial se...
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