H2O2 scavenging inhibits G1/S transition by increasing nuclear levels of p27KIP1
- Ibañez, Irene Laura
- Policastro, Lucia Laura
- Tropper, Ivanna
- Bracalente, María Candelaria
- Palmieri, Mónica A.
- Rojas, Paola Andrea
- Molinari, Beatriz Lucia
- Duran, Hebe Alicia
DOIAbstract
The aim of the present study was to evaluate cell cycle regulation by scavenging H(2)O(2) in tumor cells. A significant arrest in the G1 phase of the cell cycle was demonstrated in CH72-T4 carcinoma cells exposed to catalase, associated with a decrease in cyclin D1 and an increase in the CDK inhibitory protein p27(KIP1). Moreover, we found a differential intracellular distribution of p27(KIP1), which remained in the nucleus after catalase treatment. In vivo experiments showed an increase in nuclear levels of p27(KIP1) associated with the inhibition of tumor growth by H(2)O(2) scavenging, confirming in vitro results. To conclude, H(2)O(2) scavenging may induce cell cycle arrest through the modulation of cyclin D1 and p27(KIP1) levels and nuc...
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