Evaluation of an automated chemiluminescent immunoassay for salivary cortisol measurement. Utility in the diagnosis of Cushing's syndrome
- Repetto, Esteban Martín
- Gonzalez, Diego Javier
- Jacobsen, Dario
- Smithuis, Fernando
- Jamardo, Juan
- Cano, Mariel
- Aranda, Claudio
- Oneto, Adriana
- Berg, Gabriela Alicia
- Fabre, Bibiana
DOIAbstract
The aim of the current study was to validate an automated chemiluminescent immunoassay for cortisol quantification in saliva and to determine the cut-off level for Cushing´s Syndrome diagnosis in an adult population. The salivary cortisol levels from healthy individuals and patients previously diagnosed with Cushing´s Syndrome were measured with Siemens Immulite 2000 ® automatic chemiluminescent enzyme-immunoassay analyser with a brief modification. The limit of quantification was 3,4 nmol/L. The assay also demonstrated a high level of accuracy, as determined by linearity under dilution and recovery tests. Using this methodology, levels higher than 5.7 nmol/L is indicative of Cushing´s Syndrome with a diagnostic sensitivity and specificity ...
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