Spo0A links de novo fatty acid synthesis to sporulation and biofilm development in Bacillus subtilis
- Pedrido, María Eugenia
- de Oña, Paula
- Ramirez, Walter Joel
- Leñini, Cecilia Andrea
- Goñi, Anibal Juan
- Grau, Roberto Ricardo
DOIAbstract
During sporulation in Bacillus subtilis, the committedcell undergoes substantial membrane rearrangements to generate two cells of different sizes and fates: the mother cell and the forespore. Here, we demonstrate that the master transcription factor Spo0A reactivates lipid synthesis during development. Maximal Spo0Adependent lipid synthesis occurs during the key stages of asymmetric division and forespore engulfment. Spo0A reactivates the accDA operon that encodes the carboxylase component of the acetylCoA carboxylase enzyme, which catalyses the first and rate-limiting step in de novo lipid biosynthesis, malonyl-CoA formation. The disruption of the Spo0Abinding box in the promoter region of accDA impairs its transcriptional reactivation and...
Add to ORKG