In Vivo Anti-inflammatory Activity and Flavonoid Identification in Medicinal Eupatorium Species

Eupatorium subhastatum, E.laevigatum, E. macrocephalum, E. hecatanthum and E. candolleanum are used in traditional medicine for the treatment of ailments related to inflammation. Their aqueous and dichloromethane extracts were evaluated for in vivo anti-inflammatory activity in the carrageenan-induced paw oedema and in the TPA-induced ear oedema tests. Dichloromethane extracts of the five species significantly inhibited the mouse ear oedema, being E. subhastatum, E. laevigatum, and E. hecatanthum the most active ones, with maximum inhibitions of 86.4, 89.7, 94.4% and ED50 of 0.35, 0.51 and 0.92 mg/ear, respectively. The dichloromethane and aqueous extracts of E. subhastatum were the only active ones in the carrageenan-induced paw edema assay, both producing a moderate oedema inhibition of about 37%. No acute toxicity was observed for any of the aqueous extracts at a dose of 2g/kg. DAD-HPLC analysis of the five active dichloromethane extracts led to the identification of the anti-inflammatory compounds jaceosidin, nepetin, hispidulin and/or eupatilin in these species. The presence of these active compounds in the DCM extracts could justify at least in part their anti-inflammatory activity and the medicinal uses of these species for the treatment of local inflammations.Fil: Clavin, María L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Redko, Flavia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Acevedo, Cristina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Martino, Virginia Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Gorzalczany, Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentin