Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
- Vela Gurovic, Maria Soledad
- Gentili, Alejandro Raúl
- Olivera, Nelda Lila
- Rodriguez, Maria Susana
DOIAbstract
The production of conjugated linoleic acid (CLA) by four strains of lactic acid bacteria isolated from fish, i.e., Leuconostoc mesenteroides H20, Leuconostoc mesenteroides H22, Leuconostoc lactis H24 and Lactobacil- lus pentosus H16, was evaluated in MRS broth and on MRS agar. The bioconversion and production of CLA by resting cells were also assessed. Linoleic acid was detected in cultures grown on agar at per- centages of up to 18.3% (w/w) of total fatty acid, and conjugated isomers were found in the fatty acid profiles of Lactobacillus pentosus H16. The percentage of CLA relative to total fatty acid increased from 5.68 ± 1.65% to 23.69 ± 0.79% when resting cells were removed from agar plates and incubated with- out the addition of exogen...
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