Subject detection is a crucial task for camera-based remote healthcare monitoring. Most existing methods in subject detection rely on supervised learning of physical appearance features. However, their performances are highly restricted to the pre-trained appearance model while still suffering from false detection of human-similar objects. In this paper, we propose a novel unsupervised method to detect alive subject in a video using physiological features. Our basic idea originates from the observation that only living skin tissue of a human presents pulse-signals, which can be exploited as the feature to distinguish human skin from non-human surfaces in videos. The proposed VPS method, named Voxel-Pulse-Spectral, consists of three steps: i...