High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei
- Girault, G.
- Wattiau, P.
- Saqib, M.
- Martin, B.
- Vorimore, F.
- Singha, H.
- Engelsma, M.
- Roest, H.J.
- Spicic, S.
- Grunow, R.
- Vicari, N.
- Keersmaecker, De, S.C.J.
- Roosens, N.H.C.
- Fabbi, M.
- Tripathi, B.N.
- Zientara, S.
- Madani, N.
- Laroucau, K.
DOIAbstract
Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.</p
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