Characterization of reversed-phase liquid chromatography stationary phases for the analysis of basic pharmaceuticals: eluent properties and comparison of empirical test methods

The reversed-phase liq. chromatog. anal. of basic pharmaceuticals can be problematic. Both the properties of the eluent and the stationary phase can influence the chromatog. performance. Therefore selection of suitable exptl. conditions for the anal. of basic compds. can be difficult. This paper shows that the org. modifier and the nature of the buffer influence the eluent properties. Moreover, the nature and amt. of modifier also influence the basicity of the analytes. Investigations showed that the nature of the buffer can have a significant influence on retention and peak shape of basic compds. Test procedures using basic analytes as test probes provided relevant information with respect to selecting columns to analyze basic pharmaceutical compds. Test procedures using compds. like aniline, phenol and benzene were found to be less suitable. [on SciFinder (R)