Učinkovitost koštanoga morfogenetskoga proteina BMP1-3 u procesu cijeljenja kosti

Members of the astacin superfamily of zinc-dependent metalloproteinases regulate morphogenesis by processing precursors to mature functional extracellular matrix proteins as well as several growth factors such as TGFβ, BMP2, BMP4 and GFD8. It has been recently discovered that BMP1-3 isoform of the Bmp-1 gene circulates in the human plasma and is significantly increased in acute bone fractures. Circulating BMP1-3 regulates collagen deposition in the process of bone callus formation. In this paper role of BMP1-3 in the process of bone healing in vivo and in vitro has been evaluated. When administred systemically to rats with a femur fracture and locally to rabbits with critical size defect of ulna, recombinant BMP1-3 enhanced healing. On the opposite side, neutralization of the BMP1-3 by specific polyclonal antibody resulted in a delayed union or bone non-union. In vitro, BMP1-3 increased the expression of collagen type I and osteocalcin in MC3T3E1 osteoblast-like cells and enhanced mineralized bone modules formation from bone marrow mesenchymal stem cells. This paper shows the importance of BMP1-3 in the process of bone healing and its potential as a novel bone biomarker. By developing BMP1-3 specific assays we could monitor specific variations of its level in plasma, which could reflect on the possibility of a wide local implemantation of BMP1-3 in combinantion with other morphogens