Determination of Extracellular Hydrolytic Enzyme Production Capabilities of Acidithiobacillus Bacteria Isolated from Awasppi River in Sulaymaniyah, Iraq and 16S rDNA Analysis

In this study, the phylogenetic positions of the isolated Acidithiobacillus bacteria based on the determination of some hydrolytic extracellular enzyme activities and the sequence analysis of the 16S rDNA gene regions were determined which formed water and mud samples from the Awasspi river in Sulaymaniyah province, northern Iraq, and from the Darzilais sulphur based cave in Sangaw district. While the catalase activity of the isolates was positive, lipase, protease, xylanase and amylase activity were found to be negative. Genomic DNA’s have been isolated, 16S rDNA gene region has been realized with PCR amplification. To be able to define the distance between species Maximum Likelihood algorithm has been used to form phylogenetic tree. It has been observed that gene strands belong to Acidithiobacillus species which has been acquired from NCBI and 2 isolates that we used in our study clustered with a strong homology and their genetic positions has been revealed. In the result of the comparative 16S rDNA gene sequence analysis, it was identified that G1 isolate showed 99% similarity to Acidithiobacillus thiooxidans and G2 isolate showed 99% similarity to Acidithiobacillus ferrooxidans