The Role of BK Potassium Channels in Analgesia Produced by Alpha-2 Adrenergic Receptors

BACKGROUND AND OBJECTIVE: Millions of people suffer from pain worldwide, and annually, great economic costs are imposed on societies for pain relief. Analgesics such as alpha-2 adrenergic receptor agonists, which have low risk of complications, can be effective in assuaging pain and reducing costs. According to former studies, potassium channels play an important role in the analgesic mechanism of these receptors. This study aimed to determine the role of BK potassium channels in analgesia induced by alpha-2 adrenergic receptors. METHODS: This study was performed on 56 male Wistar rats weighing 250-300 g that were divided into seven groups of eight rats. We administered 0.7 mg/kg intraperitoneal (IP) injection of clonidine, 1 mg/kg IP injection of yohimbine, and 5 mg/kg intracerebroventricular (ICV) injection of yohimbine. Iberiotoxin at a dose of 100 nm was also injected ICV. Normal saline and DMSO were applied as solvents. Pain severity was evaluated using formalin test at a concentration of 2%. FINDINGS: The chronic pain induced by formalin injection was relieved by IP injection of 0.7 mg/kg clonidine. Moreover, 5 μg/kg and 1 μg/kg ICV administration of yohimbine with mean chronic pain scores of 2.29±0.13 and 2.09±0.07, respectively, could significantly inhibit analgesic effect of clonidine with mean chronic pain score of 1.55±0.14 (p