In this article we report the identification of a homozygous lethal T-DNA (transfer DNA) line within the coding region of the At1G05290 gene in the genome of Arabidopsis thaliana (Arabidopsis) line, SALK_063500. The T-DNA insertion is found within exon one of the AT1G05290 gene, however a homozygous T-DNA allele is unattainable. In the heterozygous T-DNA allele the expression levels of AT1G05290 were compared to wild type Arabidopsis (Col-0, Columbia). Further analyses revealed an aberrant silique phenotype found in the heterozygous SALK_063500 plants that is attributed to the reduced rate of pollen tube germination. These data are original and have not been published elsewhere. Keywords: Arabidopsis, T-DNA, SALK_063500, Silique, Pollen, Ph...
<div><p></p><p>The SALK_135513 line of <i>Arabidopsis thaliana</i> is annotated by GenBank to have t...
<p>(A) Location of the mutagenic T-DNA insertion in the 5′ UTR region of the <i>AtGNTL</i> gene. Pos...
Funder: Universität Bielefeld (3146)Abstract: Background: Experimental proof of gene function assign...
Plant genomic resources harbouring gain-of-function mutations remain rare, even though this type of ...
<div><p>T-DNA insertion mutants are a tool used widely in <em>Arabidopsis thaliana</em> to disrupt g...
Large collections of insertional Arabidopsis lines are valuable for research on functional genomics....
Callose is a plant polysaccharide that surrounds pollen mother cells and separates developing micros...
With one of the smallest known genomes for a diploid flowering plant and conservation of genetic cod...
The SALK_135513 line of Arabidopsis thaliana is annotated by GenBank to have the T-DNA insertion in ...
Contains fulltext : 27018.pdf (publisher's version ) (Open Access)Sexual plant rep...
As a strategy for the identification of T-DNA-tagged gametophytic mutants, we have used T-DNA insert...
<p>(A) The gene structure of <i>At4g33440</i> and T-DNA insertion sites of two allelic mutants. The ...
T-DNA flanking sequences were isolated from 112 Arabidopsis thaliana single-copy T-DNA lines and seq...
To assist in the analysis of plant gene functions we have generated a new Arabidopsis insertion muta...
The SALK_135513 line of Arabidopsis thaliana is annotated by GenBank to have the T-DNA insertion in...
<div><p></p><p>The SALK_135513 line of <i>Arabidopsis thaliana</i> is annotated by GenBank to have t...
<p>(A) Location of the mutagenic T-DNA insertion in the 5′ UTR region of the <i>AtGNTL</i> gene. Pos...
Funder: Universität Bielefeld (3146)Abstract: Background: Experimental proof of gene function assign...
Plant genomic resources harbouring gain-of-function mutations remain rare, even though this type of ...
<div><p>T-DNA insertion mutants are a tool used widely in <em>Arabidopsis thaliana</em> to disrupt g...
Large collections of insertional Arabidopsis lines are valuable for research on functional genomics....
Callose is a plant polysaccharide that surrounds pollen mother cells and separates developing micros...
With one of the smallest known genomes for a diploid flowering plant and conservation of genetic cod...
The SALK_135513 line of Arabidopsis thaliana is annotated by GenBank to have the T-DNA insertion in ...
Contains fulltext : 27018.pdf (publisher's version ) (Open Access)Sexual plant rep...
As a strategy for the identification of T-DNA-tagged gametophytic mutants, we have used T-DNA insert...
<p>(A) The gene structure of <i>At4g33440</i> and T-DNA insertion sites of two allelic mutants. The ...
T-DNA flanking sequences were isolated from 112 Arabidopsis thaliana single-copy T-DNA lines and seq...
To assist in the analysis of plant gene functions we have generated a new Arabidopsis insertion muta...
The SALK_135513 line of Arabidopsis thaliana is annotated by GenBank to have the T-DNA insertion in...
<div><p></p><p>The SALK_135513 line of <i>Arabidopsis thaliana</i> is annotated by GenBank to have t...
<p>(A) Location of the mutagenic T-DNA insertion in the 5′ UTR region of the <i>AtGNTL</i> gene. Pos...
Funder: Universität Bielefeld (3146)Abstract: Background: Experimental proof of gene function assign...