Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition

The conjugation of maleimide reporter groups to 5′‐diene‐modified oligonucleotides via aqueous Diels‐Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene‐amidite (5′‐diene modifier), for the attachment of a diene group to the 5′‐terminus of oligonucleotides, and for the conjugation of maleimide reporter groups to diene‐modified oligonucleotides.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143620/1/cpnc0418.pd