Studies on the effect of ethanolic extract of propolis in BALB/c mice

Propolis is widely used since ages for the treatment of various ailments. Present study focussed on the toxicity profile of ethanolic extract of propolis on BALB/c mice. The effect of different concentrations of propolis (300, 500, 1000 mg/kg body weight) was analysed by studying the biochemical, haematological and histological changes in mice for 28 days. No significant difference in various parameters were observed in groups of mice treated with propolis and the normal control (p>0.05). Histological findings on liver, spleen, kidney and brain revealed normal architecture. The ethanolic extract of propolis did not produce significant toxic effect in mice and hence can be utilized for nutraceuticals formulations