The theme of this thesis concerns the fundamental questions in soft matter, in particular, the dynamics of vesicle membranes, interfacial polymers, colloidal particles. Optical imaging was applied three distinct systems to reveal the underlying physical processes that were hindered by other prior indirect measurements. First, mechanical responses of both polymer and lipid vesicles were investigated by watching their shape transformations under environmental variations, mostly under osmotic shocks, which emitted new prospects on membrane elasticity, instability and kinetics. Furthermore, with statistical analysis of particle and pattern tracking, three classical questions in polymer physics regarding polymer-surface interactions were revisit...