Phenobarbital Regulation of CYP2B2, Cyp2b10, and Cyp2b9

129 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001.A new in vivo protocol, mouse tail vein injections, was developed to study PB induction of the mouse Cyp2b10 and Cyp2b9 PBRUs. In this model, the Cyp2b10 PBRU, which is 91% similar to the CYP2B2 PBRU, was induced by PB. A construct harboring the Cyp2b9 PBRU, which is 73% similar to the Cyp2b10 PBRU, was not PB responsive. Analysis of the PB responsiveness of Cyp2b10 PBRU mutants showed that Cyp2b10 and CYP2B2 may share the same mechanism of PB induction in which NR1 and NF-1 are the most important elements. The GRE-like and AF-1 elements also participate in the PB induction of Cyp2b10.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD