The programmed destabilisation of centrioles in the Drosophila egg chamber through the APC/C ubiquitination pathway

Resumen del póster presentado a la 25th European Drosophila Research Conference, celebrada en Londres (UK) del 22 al 25 de septiembre de 2017.Centrioles are organelles that are present in most of animal cells and are often part of a larger complex called the centrosome, which is composed of the centrioles and pericentriolar material (PCM). The centrosomes regulate various cellular processes, including cell division and establishing cell polarity through their microtubule organising capabilities. In particular, centrosomes are necessary for ciliogenesis and asymmetric cell divisions. In the Drosophila melanogaster egg chamber centriole elimination is thought to prevent parthenogenesis, due to a reticulate mechanism of egg activation prior to fertilisation. lf the centrioles are not eliminated during Drosophila female germline development then the eggs are sterile. However, the centrioles of the germline not only have to be eliminated, but must undergo a peculiar orchestrated relocalisation to the oocyte prior to this elimination. Therefore, centrioles must be strictly controlled for Drosophila fertility. Despite the importance of centriole maintenance in the female germline, the mechanism of this germline-specific regulation is still nebulous. With a modified version of the anaphase promoting complex/cyclosome (APC/C), we show that precise control of the APC/C is responsible for centriole/centrosome regulation during Drosophila oogenesis. The APC/C regulates the programmed destruction of various cell cycle proteins, and we show that in the female germline this includes Polo, a key regulator necessary for PCM and thus centriole stability. lt is through the specifically timed destruction of Polo that the APC/C enables germline centriole elimination. In addition to this, we show the developmental consequence of ectopic centriole regulation.Peer Reviewe