Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody(MabB23) specific for human plasma apolipoprotein B-100

We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23 (γ2b,λ), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and λ light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.ope