Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification

This thesis report is submitted in partial fulfilment of the requirement for the degree of M.Sc. in Biotechnology, 2018.Catalogued from PDF version of thesis report.Includes bibliographical references (pages 48-52).The cellulase producing bacteria were isolated from cow dung and characterized by morphological and biochemical analysis. Hence, studies pursue to unfold a novel cellulase that can overcome existing challenges in biorefinery, reduce biofuel production cost, as well as have tremendous applications in industrial processes. Further partial purification of cellulase was carried out by dialysis and ammonium sulphate precipitation. The partially purified enzyme was then purified by gel filtration chromatography using sephadex G-75 matrix and also to determine molecular weight by SDS-PAGE. Bacterial isolate were grown on Carboxy Methyl Cellulose (CMC) agar at various optimum conditions such as parameters like pH, temperature, incubation period prior to examine. In the present study, the result showed that cellulase producing bacteria can grow at optimized condition. The best isolate so far had an extracellular crude enzyme activity of 0.076 U/ml. The use of microorganisms for the production of enzymes offers a promising approach for its large scale production and as a possible food supplement or in the pharmaceutical industry.M. Biotechnolog