Additional file 1: of Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells

Figure S1. Control of customized free fatty acid receptor 4 (FFAR4) antibody specificity. Protein extracts from bovine lung tissue were separated by electrophoresis on 4–12% (w:v) SDS-polyacrylamide gel. After electrotransfer to nitrocellulose membranes, the proteins were probed with anti-FFAR4 antibody (0.95 μg/mL, customized FFAR4 rabbit antibody, Agro-Bio), which was pre-incubated for 15 min with different concentrations of the bovine specific peptide (Agro-Bio) used to produce the antibody (from 0 to 2.5 μg/mL). The blots were stripped and re-probed with antibodies against vinculin (VCL) used as the loading control. (TIF 93 kb