F-actin dynamics in the <i>pgkc-1</i> mutant.

<p><b>(A)</b> Lifeact-mEGFP signal in WT and <i>pgkc-1</i> pollen tubes with mock or 1.5 nM LatB treatment. Scale bar = 5 μm. <b>(B)</b> Average GFP signal intensity along WT and <i>pgkc-1</i> pollen tubes with mock or 1.5 nM LatB treatment. Measurements were performed as described in Materials and Methods. Thirty-five pollen tubes were measured for each sample. The 0 μm indicates the position of the extreme tip. Orange line indicates WT pollen tube; red line indicates WT pollen tube treated with 1.5 nM LatB; gray line indicates <i>pgkc-1</i> pollen tube; black line indicates <i>pgkc-1</i> pollen tube treated with 1.5 nM LatB. Error bars on curves indicate standard error of the mean. <b>(C and D)</b> WT and <i>pgkc-1</i> pollen tube growth when subjected to mock medium <b>(C)</b> or 1.5 nM LatB <b>(D)</b> treatment. Scale bar = 50 μm. <b>(E to G)</b> WT and <i>pgkc-1</i> plant pollen germination <b>(E)</b>, pollen tube length <b>(F)</b>, and pollen tube width <b>(G)</b> when subjected to mock or 1.5 nM LatB treatment. Bars represent mean ± SEM. Asterisks indicate significant differences versus mock treatment as determined using Student’s <i>t</i>-test (** = p < 0.001).