Effects of disrupting GAPDH on pollen tube polarity.

<p><b>(A)</b> Glycolytic pathway of GAPDH and PGK. <b>(B to G)</b> Pollen tube morphology of WT <b>(B)</b>, <i>pgkc-1</i> <b>(C)</b>, and <i>ren1-3</i> <b>(D)</b> plants subjected to mock treatment; pollen tube morphology of WT <b>(E)</b>, <i>pgkc-1</i> <b>(F)</b>, and <i>ren1-3</i> <b>(G)</b> plants treated with 40 μM CGP 3466B. Both <i>pgkc-1</i> and <i>ren1-3</i> plants were dramatically depolarized by CGP medium. Scale bar = 50 μm. <b>(H and I)</b> Pollen tube length <b>(H)</b> and width <b>(I)</b> of WT, <i>pgkc-1</i>, and <i>ren1-3</i> pollen tubes subjected to mock and 40 μM CGP treatment. Bars represent mean ± SEM. Asterisks indicate significant differences versus either single mutant as determined using Student’s <i>t</i>-test with either single mutant (** = p < 0.001). <b>(J)-(M)</b> Average signal intensity along WT pollen tubes subjected to mock or CGP treatment of <b>(J)</b> GFP- REN1, <b>(K)</b> CRIB4-GFP, <b>(L)</b> Lifeact-mEGFP, <b>(M)</b> EYFP-RABA4D. Measurements were performed as described in Materials and Methods. Fifteen pollen tubes from each sample were measured. The 0 μm label indicates the position of the apical tip.