Vesicle trafficking in the <i>pgkc-1</i> mutant.

<p><b>(A)</b> EYFP-RABA4D signal in WT and <i>pgkc-1</i> pollen tubes subjected to mock or 0.4 μM BFA treatment. Scale bar = 5 μm. <b>(B)</b> EYFP-RABA4D signal intensity. Measurements were performed as described in Materials and Methods. Fifteen to twenty pollen tubes from each sample were measured. 0 μm indicates the position of apical tip. Error bars on curves indicate standard error. <b>(C and D)</b> WT and <i>pgkc-1</i> pollen tube morphology when subjected to <b>(C)</b> mock and <b>(D)</b> 0.4 μM BFA treatment. Scale bar = 50 μm. <b>(E to G)</b> WT and <i>pgkc-1</i> plant pollen germination <b>(E)</b>, pollen tube length <b>(F)</b>, and pollen tube width <b>(G)</b> when subjected to mock or 0.4 μM BFA treatment. Bars represent mean ± SEM. Asterisks indicate significant differences versus mock treatment as determined using Student’s <i>t</i>-test (** = p < 0.001).