ROP1 signaling in the <i>pgkc-1</i> mutant.

<p><b>(A)</b> Active ROP1 visualized by CRIB4-GFP signal in WT and <i>pgkc-1</i> pollen tubes. Scale bar = 5μm. <b>(B)</b> Average CRIB4-GFP signal intensity along WT and <i>pgkc-1</i> pollen tubes. <b>(C)</b> GFP-REN1 localization in WT and <i>pgkc-1</i> pollen tubes. Scale bar = 5 μm. <b>(D)</b> Average GFP-REN1 signal intensity along WT and <i>pgkc-1</i> pollen tubes. Measurements were performed as described in Materials and Methods. Fifteen pollen tubes from each sample were measured. The 0 μm label indicates the position of the apical tip. Error bars on curves indicate standard error of the mean. <b>(E to G)</b> Pollen tube morphology of <i>pgkc-1</i> <b>(E)</b>, <i>ren1-3</i> <b>(F)</b>, and <i>ren1-3/pgkc-1</i> double mutant plants <b>(G)</b>. Scale bar = 50 μm. <b>(H and I)</b> Pollen tube length <b>(H)</b> and width <b>I)</b> of WT, <i>pgkc-1</i>, <i>ren1-3</i>, and <i>ren1-3/pgkc-1</i> double mutant plants. Bars represent mean ± SEM. Asterisks indicate significant differences versus single mutant plant as determined using Student’s <i>t</i>-test (** = p < 0.001).