Adult hemocytes quantification and phagocytosis capability.

<p>(A) Confocal microscopy image of a fly with genetically labeled Hml>GFP hemocytes (green) and injected with pHrodo, which fluoresces red after being phagocytosed; yellow coloration marks co-localization of green Hml>GFP positive cells being able to phagocytose the red pHrodo. White line labels the area of side thorax were hemocytes were counted. (B) Number of Hml>GFP positive cells and pHrodo phagocytosing cells (and their combinations) in <i>w</i> control (black) and the <i>adoR</i> (red) and <i>adgf-a/+</i> (green) mutant flies. Cells were counted in the area marked in (A). Neither mutant genotype affects the total number of hemocytes or the basal capacity of phagocytosis. (C) Number of Hml>GFP and pHrodo-positive cells in flies infected by <i>S</i>. <i>pneumoniae</i> 6 hours prior to pHrodo injection. While there is comparable number of Hml>GFP cells upon infection in all genotypes, the infected <i>adoR</i> mutant shows a lower number of phagocytic cells with a carbohydrate-poor, 0%-glucose diet, which is improved by a carbohydrate-rich, 10%-glucose diet (purple). The number of cells was counted in the same area as depicted in (A). Dots in graphs show individual counts, with lines marking the mean values; asterisks mark significantly different values determined by t-tests.