3D-2C PIV measurements throughout the heartbeat of a ~3 day old zebrafish heart

Depth-resolved (3D-2C) Particle Image Velocimetry analysis results using OpenPIV on Selective Plane Illumination Microscopy data of the flow of fluorescent Red Blood Cells (gata:DsRed) in a ~3 days post fertilization zebrafish embryo heart (focus on the atrium). Flow results obtained using correlation averaging by using optical gating techniques. A single plane of raw data is shown to aid visualization. The heart beat is divided into 31 equally sized phase bins in the range of (0,2*pi). The vectors are scaled to the square root of their magnitudes to better reveal the dynamic range of the blood flow velocities. Acquisition parameters: Laser pulse length: 1ms Inter-pulse time: 1.5ms z-step: 8um Camera pixel binning: 2x2 Effective pixel size (at 20x): 0.3225um OpenPIV parameters: Small Interrogation Window size (LxH): 24x24 Big Interrogation Window size (LxH): 72x48 Peak-to-peak threshold: 1.07 Mask size: 7x7 pixels Animals were maintained according to the Animals (Scientific Procedures) Act 1986, United Kingdom. The fish were the larvae were anaesthetized with 168mg/L solution of Tricaine Methanesulfonate throughout the experiments. Visualized with Mayavi